Quantitative Measurement of microRNAs Reveals Distinct Correlation with Acute Myeloid Leukemia (AML) Signature
Poster Sep 30, 2005
Silvana Debernardi, Spyros Skoulakis, Gael Molloy, Bryan D. Young
IntroductionMicroRNAs (miRNAs) are short single stranded RNAs that have a potentially important role in gene regulation. That role is due to complementarity mediated binding to target mRNAs resulting in the repression of translation and in the cleavage of the target transcript. There are indications that miRNAs might be a new class of genes involved in human tumorigenesis.
Using a novel quantitative real-time PCR assay, specific to the mature product, the expression level of a selected group of miRNAs was measured across a set of 30 primary adult acute myeloid leukemia (AML) with a normal karyotype. Calculation of the correlation between miRNA levels and the genome-wide AML expression profile identified distinct patterns of miRNA expression that correlate with the AML signature.
In addition, the genes whose expression was most significantly associated with each miRNA were identified. This is the first study reporting a quantitative measurement of mature miRNA expression and the computational discovery of genome-wide associations in primary malignancies.
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
Novel Role of the Innate Immune DNA Sensor IFI16 (Interferon Gamma Inducible Protein 16) as a Major Epigenetic Modulator During KSHV Infection and Lytic ReactivationPoster
Studies have shown that IFI16 acts as an antiviral restriction factor against a number of DNA viruses, by inhibiting viral replication or transcription through epigenetic modifications. However, till date, no specific epigenetic function of IFI16 has been identified. Here, we have discovered that IFI16 recruits two histone methyltransferases on the KSHV episome leading to altered Histone H3K9 methylation, thus regulating its lifecycle.READ MORE
A new method for generating arrayed RNAi screening tools for any organismPoster
RNA interference (RNAi) using small interfering RNAs (siRNAs) is an important technology for down-regulation of gene expression and a powerful tool to study cellular processes and pathways. Previously, large collections of siRNAs were available only for traditional experimental model systems, such as human and mouse, and predominantly provided as chemically synthesized libraries.READ MORE