Quantitative Measurement of microRNAs Reveals Distinct Correlation with Acute Myeloid Leukemia (AML) Signature
Poster Sep 30, 2005
Silvana Debernardi, Spyros Skoulakis, Gael Molloy, Bryan D. Young
IntroductionMicroRNAs (miRNAs) are short single stranded RNAs that have a potentially important role in gene regulation. That role is due to complementarity mediated binding to target mRNAs resulting in the repression of translation and in the cleavage of the target transcript. There are indications that miRNAs might be a new class of genes involved in human tumorigenesis.
Using a novel quantitative real-time PCR assay, specific to the mature product, the expression level of a selected group of miRNAs was measured across a set of 30 primary adult acute myeloid leukemia (AML) with a normal karyotype. Calculation of the correlation between miRNA levels and the genome-wide AML expression profile identified distinct patterns of miRNA expression that correlate with the AML signature.
In addition, the genes whose expression was most significantly associated with each miRNA were identified. This is the first study reporting a quantitative measurement of mature miRNA expression and the computational discovery of genome-wide associations in primary malignancies.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
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