Rapid analysis of 3D tumour spheroids in soft agar and on ultra-low attachment plates using a laser scanning imaging system
Poster Feb 19, 2014
Anne F Hammerstein, Diana Caracino, and Paul Wylie
Research to identify new anticancer drugs is currently facing significant challenges, as only 5% of compounds that show efficacy in pre-clinical development go on to become licensed drugs. Traditionally 2D cell culture models have been employed to evaluate drug candidates in the early phases of the drug discovery process, however, there is increasing evidence that cells grown in 2D monolayers do not accurately reflect the biological complexity of tumours. The requirement for better in vitro tumour models that are compatible with high throughput screening campaigns has led to the development of 3D cell cultures models, especially muliticellular tumour spheroids, which retain many of the morphological and genetic traits of tumours.
Here we describe the formation of such spheroids by two methods: On ultra-low attachment plates and in semi-solid agarose. Both methods are compatible with 96- and 384-well microplate formats. We then used the acumen cellista to rapidly image entire microplates (<5 minutes/plate), reporting a range of parameters such as spheroid number, area and volume. The acumen cellista is ideally suited to the high content analysis of spheroids, as the whole-well scanning capability of the instrument will include data from all the spheroids in a well, while the large depth of field of the scan lens allows the determination of individual spheroid volume without the need to acquire a Z stack of images.
P450 Induction in Cryopreserved Hepatocytes from PXR and CAR Nuclear Receptor Knock-out RatsPoster
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE
Regulatory T-Cells (Tregs) Within Bone Marrow-Derived Stem Cells (BMSCs) Actively Confer Immunomodulatory and Neuroprotective Effects Against StrokePoster
We found a distinct subpopulation of Tregs within BMSCs. Tregs and BMSCs in co-culture conferred neuroprotection that varied in a dose-dependent manner. Tregs minimized stem cell production of IL-6, a pro-inflammatory cytokine, and inhibited BMSC secretion of FGF-beta, a cytokine related to BMSC proliferation and differentiation. The ratio of Tregs found natively in BMSCs is optimally adapted to provide the maximum neuroprotective benefit of stem cell treatment after ischemic stroke.READ MORE
Internet-Based Biomarker Collection Feasibility: Experiences of a Tobacco Cessation Program for People Living with HIVPoster
This project introduces video-conferencing as a method for biomarker collection in research and will explain how biomarker collection via video-conferencing was implemented in a pilot ehealth intervention.READ MORE