Rapid analysis of 3D tumour spheroids in soft agar and on ultra-low attachment plates using a laser scanning imaging system
Poster Feb 19, 2014
Anne F Hammerstein, Diana Caracino, and Paul Wylie
Research to identify new anticancer drugs is currently facing significant challenges, as only 5% of compounds that show efficacy in pre-clinical development go on to become licensed drugs. Traditionally 2D cell culture models have been employed to evaluate drug candidates in the early phases of the drug discovery process, however, there is increasing evidence that cells grown in 2D monolayers do not accurately reflect the biological complexity of tumours. The requirement for better in vitro tumour models that are compatible with high throughput screening campaigns has led to the development of 3D cell cultures models, especially muliticellular tumour spheroids, which retain many of the morphological and genetic traits of tumours.
Here we describe the formation of such spheroids by two methods: On ultra-low attachment plates and in semi-solid agarose. Both methods are compatible with 96- and 384-well microplate formats. We then used the acumen cellista to rapidly image entire microplates (<5 minutes/plate), reporting a range of parameters such as spheroid number, area and volume. The acumen cellista is ideally suited to the high content analysis of spheroids, as the whole-well scanning capability of the instrument will include data from all the spheroids in a well, while the large depth of field of the scan lens allows the determination of individual spheroid volume without the need to acquire a Z stack of images.
IntelliXtract 2.0: Simplified Intelligent Component Extraction and DetectionPoster
*Simplified detection and Component Extraction algorithm from LC-MS and GC-MS datasets
*New improved algorithm based on ion threads
*Reduced number of parameters to select for analysis
*Reduced false positives leading to reduced analysis time
Designing a Model to Explore Tau's Unfolded Protein ResponsePoster
The purpose of this research is to design a cell model in which ER stress caused by tau accumulation can be generated, and then investigated for changes in different ER stress-associated proteins.READ MORE
A New Method for Analyzing MSe/All Ions Fragmentation in Xenobiotic Metabolism StudiesPoster
During early drug discovery, the study of metabolism plays an essential role in determining which drug candidates move forward into development and later stages. As an alternative to traditional Data Dependent Acquisition (DDA), the use of MSE/All Ions Fragmentation (AIF) has become common in metabolite identification workflows for the analysis of metabolic hot spots. Here we present a solution for analysis of MSE/AlF in metID studies.READ MORE