Rapid analysis of 3D tumour spheroids in soft agar and on ultra-low attachment plates using a laser scanning imaging system
Poster Feb 19, 2014
Anne F Hammerstein, Diana Caracino, and Paul Wylie
Research to identify new anticancer drugs is currently facing significant challenges, as only 5% of compounds that show efficacy in pre-clinical development go on to become licensed drugs. Traditionally 2D cell culture models have been employed to evaluate drug candidates in the early phases of the drug discovery process, however, there is increasing evidence that cells grown in 2D monolayers do not accurately reflect the biological complexity of tumours. The requirement for better in vitro tumour models that are compatible with high throughput screening campaigns has led to the development of 3D cell cultures models, especially muliticellular tumour spheroids, which retain many of the morphological and genetic traits of tumours.
Here we describe the formation of such spheroids by two methods: On ultra-low attachment plates and in semi-solid agarose. Both methods are compatible with 96- and 384-well microplate formats. We then used the acumen cellista to rapidly image entire microplates (<5 minutes/plate), reporting a range of parameters such as spheroid number, area and volume. The acumen cellista is ideally suited to the high content analysis of spheroids, as the whole-well scanning capability of the instrument will include data from all the spheroids in a well, while the large depth of field of the scan lens allows the determination of individual spheroid volume without the need to acquire a Z stack of images.
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A femoral nerve defect model was adapted for the evaluation of proregenerative effects of extracorporeal shockwave therapy (ESWT). Functional evaluation, histology and qRT-PCR data show differences between sensory and motor-derived nerve transplants and a pro-regenerative effect of ESWT. These data provide evidence for the clinical application of ESWT after autologous nerve transplantation as a novel non-invasive method.READ MORE
Depressive Symptoms Related to Biological Markers of Immune Functioning among Young People with HIVPoster
Youth living with HIV (YLWH) are at risk for depression. This study aimed to identify trends in depressive symptoms for YLWH in a specialty-care clinic and follow up clinical treatment procedures.READ MORE
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