Rapid and Miniaturized Robotic Crystallization Experiment using the Honeybee 963™ System
Poster Feb 13, 2006
Malcolm Willson, Hamid Khoja and Charles Dang
AbstractAn important consideration in protein crystallization screening is the limited availability of protein which makes the miniaturization of these experiments a necessity. Considerations such as dispense speed, protein concentration, protein/screening agent drop ratios, evaporation, etc. all need to be carefully analyzed when designing the crystallization experiment.
The Honeybee 963™ enables rapid, precise and accurate dispensing of nanoliter (nl) volumes of screening and protein solutions. Strategies for experimental design will be discussed within the framework of the dispensing technologies that are utilized in the Honeybee 963.
The ability to dispense up to three different proteins and/or protein concentrations coupled with the 96 tip dispensing head for screening solutions ensures evaporation is kept to a minimum and that protein usage is conserved.
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
Novel Role of the Innate Immune DNA Sensor IFI16 (Interferon Gamma Inducible Protein 16) as a Major Epigenetic Modulator During KSHV Infection and Lytic ReactivationPoster
Studies have shown that IFI16 acts as an antiviral restriction factor against a number of DNA viruses, by inhibiting viral replication or transcription through epigenetic modifications. However, till date, no specific epigenetic function of IFI16 has been identified. Here, we have discovered that IFI16 recruits two histone methyltransferases on the KSHV episome leading to altered Histone H3K9 methylation, thus regulating its lifecycle.READ MORE
A new method for generating arrayed RNAi screening tools for any organismPoster
RNA interference (RNAi) using small interfering RNAs (siRNAs) is an important technology for down-regulation of gene expression and a powerful tool to study cellular processes and pathways. Previously, large collections of siRNAs were available only for traditional experimental model systems, such as human and mouse, and predominantly provided as chemically synthesized libraries.READ MORE
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