Rapid and Miniaturized Robotic Crystallization Experiment using the Honeybee 963™ System
Poster Feb 13, 2006
Malcolm Willson, Hamid Khoja and Charles Dang
AbstractAn important consideration in protein crystallization screening is the limited availability of protein which makes the miniaturization of these experiments a necessity. Considerations such as dispense speed, protein concentration, protein/screening agent drop ratios, evaporation, etc. all need to be carefully analyzed when designing the crystallization experiment.
The Honeybee 963™ enables rapid, precise and accurate dispensing of nanoliter (nl) volumes of screening and protein solutions. Strategies for experimental design will be discussed within the framework of the dispensing technologies that are utilized in the Honeybee 963.
The ability to dispense up to three different proteins and/or protein concentrations coupled with the 96 tip dispensing head for screening solutions ensures evaporation is kept to a minimum and that protein usage is conserved.
Characterization of a Type 2 diabetes-associated islet-specific enhancer cluster in STARD10 by genome editing of EndoC-βH1 cellsPoster
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
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The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE
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Early life stress (ELS) is highly associated with development of psychopathology
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