Rapid and Miniaturized Robotic Crystallization Experiment using the Honeybee 963™ System
Poster Feb 13, 2006
Malcolm Willson, Hamid Khoja and Charles Dang
AbstractAn important consideration in protein crystallization screening is the limited availability of protein which makes the miniaturization of these experiments a necessity. Considerations such as dispense speed, protein concentration, protein/screening agent drop ratios, evaporation, etc. all need to be carefully analyzed when designing the crystallization experiment.
The Honeybee 963™ enables rapid, precise and accurate dispensing of nanoliter (nl) volumes of screening and protein solutions. Strategies for experimental design will be discussed within the framework of the dispensing technologies that are utilized in the Honeybee 963.
The ability to dispense up to three different proteins and/or protein concentrations coupled with the 96 tip dispensing head for screening solutions ensures evaporation is kept to a minimum and that protein usage is conserved.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE