Rapid Identification of Microorganisms by Touch Spray and Paper Spray Ambient Ionization
Poster Feb 26, 2014
Ahmed M. Hamid, Alan K. Jarmusch, Valentina Pirro and R. Graham Cooks
Rapid and accurate identification of bacterial and yeast isolates is needed to advance public health. Touch spray is a novel ambient ionization method developed. It is performed by using any metal probe capable of transferring material from the object of interest to the surface of the probe for analysis. In this particular application, the bacterial colonies are directly sampled from culture dishes. Touch spray ionization can also be used to detect suspended micro-organisms in various solvents, such as methanol, water, dimethyl formamide and acetonitrile. Detected lipid profiles are characteristic for various bacteria genera. Touch spray has been utilized efficiently to distinguish among gram-negative and gram-positive bacteria in both of positive mode and negative modes. As an example of species differentiation, Staphylococcus aureus, Staphylococcus hominis, and Staphylococcus epidermidis are readily distinguished through their lipid patterns utilizing multivariant statistical analyses. In a parallel study, individual bacterial colonies are transferred to a filter paper cut into a sharp point and analyzed using paper spray-MS. Comparison of the mass-spectral data obtained from paper spray and touch spray ionization techniques show high degree of similarity and reproducibility.
A New Tool for the Automated Sample Preparation of Whole Blood Samples by LC-MS using a Commercial AutosamplerPoster
Automated sample preparation reduces the costs per sample and minimizes sample handling errors. Usually expensive and highly specialized pipetting robots are used. However, most of these systems are not designed with a direct interface for LC-MS applications. In addition common pipetting systems are not optimized for smaller scale sample series. Here we present a new tool for liquid handling of whole blood samples and direct sample injection.
Multi-Residue Pesticide Analysis in Food Matrices Using Ultivo Triple Quad LC/MSPoster
Regulatory agencies have set maximum residue levels for hundreds of pesticides and their metabolites in foods.READ MORE
Analysis of Mycotoxins in Food Matrices using the Innovative Ultivo Triple Quad LC/MSPoster
A study demonstrating the accuracy and sensitive quantification of up to 12 regulated mycotoxin compounds in three commonly regulated foods.READ MORE
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