Real-time PCR Gene Expression Profiling
Poster Apr 05, 2007
Mikael Kubista, José Manuel Andrade, Björn Sjögreen and Amin Forootan
Real-time PCR has rapidly become the preferred technique for quantitative analysis of nucleic acids. Its superior sensitivity, reproducibility, and dynamic range make it the preferred choice for both absolute and relative quantification of nucleic acids, as well as expression profiling. Correct interpretation of real-time PCR data requires appropriate experimental design, accurate data pre-processing and analysis of the data using proper statistical and multivariate methods. For this process we have developed the GenEx software.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE