Real-time PCR Gene Expression Profiling
Poster Apr 05, 2007
Mikael Kubista, José Manuel Andrade, Björn Sjögreen and Amin Forootan
Real-time PCR has rapidly become the preferred technique for quantitative analysis of nucleic acids. Its superior sensitivity, reproducibility, and dynamic range make it the preferred choice for both absolute and relative quantification of nucleic acids, as well as expression profiling. Correct interpretation of real-time PCR data requires appropriate experimental design, accurate data pre-processing and analysis of the data using proper statistical and multivariate methods. For this process we have developed the GenEx software.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE