Revealing Host Metabolome Reprogramming by the Economically Important Fungal Plant Pathogens Botrytis cinerea and Magnaporthe grisea
Poster Mar 28, 2007
Aileen R., Smith J., William Allwood, Amanda J. Lloyd, David I. Ellis, Royston Goodacre and Luis A.J. Mur
Metabolomic approaches including Fourier- transform infrared (FT-IR) spectroscopy and Electrospray ionisation mass spectrometry (ESI-MS) were used to elucidate changes during susceptible and resistant interactions of Magnaporthe grisea with B. distachyon. Following data mining, discriminatory analytes (m/z) were shown to be phospholipids (PL) by ESIMS-MS. We are now moving to correlate transcriptional and metabolomic changes, concentrating on PL-based regulation.
This same Metabolomic approach is being applied to the Botrytis cinerea interaction with Arabidopsis wild-type and ethylene signalling mutants, which exhibit either enhanced or compromised defence.
We developed an automated sample preparation protocol based on a robotic platform PAL RTC (CTC Analytics AG, Zwingen Switzerland), which represent a modified Bligh and Dyer method producing samples for both hydrophilic metabolomics using GC-MS and lipidomics using SFC-MS simultaneously.READ MORE