Role of TDP-43 in activation of the brain inflammatory response
Poster Feb 13, 2017
Frank Zamudio, Corbin Rodier, Anjanet Loon, Khawla Benyamine, Carlos Osorno, Shayna Smeltzer, and Maj-Linda Selenica
TDP-43 is associated with fronto-temporal lobar dementia (FTLD) and some cases of Alzheimer’s disease. Previous studies have found a role for TDP-43 in regulation of inflammatory markers such as IL-6, but the effect of increased TDP-43 expression on inflammation has not been studied. In order to fill these gaps, we induced peripheral inflammation in WT and TDP-43 overexpressing mice and began profiling changes in inflammatory cytokines, receptors, and kinases, in addition to changes in behavior.
In order to generate a robust protocol for MEA recording on hiPSC- derived neurons, we evaluated several conditions, which could affect culture performance (1.neuron seeding density; 2.seeding medium; 3.astrocyt eco-culture). These conditions were evaluated with BrainXell’s hiPSC-derived spinal motor neurons, cortical glutamatergic neurons and mixed cortical neurons.READ MORE