rptDB; a prototype Affymetrix .rpt QC tool
Poster Sep 22, 2005
James Hadfield, Marielle Vigouroux
Affymetrix analysis results in the generation of large and complex data sets which need extensive downstream analysis to determine results of biological significance. Users can benefit from a careful QC of their data before beginning analysis to remove individual chips that may adversely affect the final interpretation.
rptDB is a prototype database for the analysis of Affymetrix expression report (.rpt) files. Report files are widely used to QC raw data after hyb, scan and .cel file generation. Only %Present calls, Scale Factors and 3':5' ratios of housekeeping genes are generally used, but there are many other metrics that may also be of importance for QC analysis.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE