Sample Stabilization and Purification Devices for Superior Forensic DNA Storage, Purification and Profiling
Mawi has developed a sample collection system called iSWAB-Forensics, which is designed to be utilized indiscriminately for both reference and evidentiary sample collection. iSWAB-Forensics enables the long term room temperature stabilization of the collected sample at the point of collection. The sample recovery from the swab is achieved by including a squeezing insert within the device that allows for the maximum release of the collected sample from a swab holding evidentiary or reference samples into a proprietary DNA stabilizing buffer.
Several reference and mocked evidentiary samples (dry blood, fresh blood, buccal, lipstick, semen, touch DNA from a water bottle, steering wheel etc.) were collected with iSWAB-Forensics and transported to BioChain utilizing the standard US postal services system. At BioChain, the samples were aliquoted in 100 µL volumes and stored at room temperature until processed. BioChain processed the samples on their automated DNA purification system, AnaPrep, utilizing their blood extraction protocol. The samples were processed 3 days and 15 days post-collection. All samples had detectable and quantifiable amounts of human gDNA (Nanodrop and Investigator Quantiplex Kit). There was insignificant human gDNA loss between samples processed 3 or 15 days post collection. The functionality of the AnaPrep purified samples were successfully analyzed with the Identifiler® kit. In conclusion, the iSWAB-Forensics is an efficient recovery and stabilization sample collection tool for both reference and evidentiary samples. Also, the AnaPrep automated extraction system can be utilized for DNA sample processing in a forensics setting.