Screening and Profiling for Kinases: Development of a Unique and Versatile Platform

Abstract

To build a unique kinase platform providing reliable and quantitative data, we have designed a flexible system with well-defined yet different assay conditions for each kinase. This platform minimizes interferences, including those from test compounds, and displays a homogeneous and generic assay format.

After reviewing available assay technologies, Homogeneous Time Resolved Fluorescence (HTRF) was selected as that which best met our requirements. To date, 70 kinase assays have been developed on this model. Using pertinent examples, we will illustrate how this technology supports and serves scientific drug discovery, and allows direct and obvious comparison across the panel for both high throughput compound profiling and high throughput screening.