Screening and Profiling for Kinases: Development of a Unique and Versatile Platform
Poster Nov 10, 2005
M. Chambon, A. Otto-Bruc, V. Hamon, C. Pernelle – Cerep, BP1, Le Bois L’Evêque, 86600 Celle L’Evescault, France
To build a unique kinase platform providing reliable and quantitative data, we have designed a flexible system with well-defined yet different assay conditions for each kinase. This platform minimizes interferences, including those from test compounds, and displays a homogeneous and generic assay format.
After reviewing available assay technologies, Homogeneous Time Resolved Fluorescence (HTRF) was selected as that which best met our requirements. To date, 70 kinase assays have been developed on this model. Using pertinent examples, we will illustrate how this technology supports and serves scientific drug discovery, and allows direct and obvious comparison across the panel for both high throughput compound profiling and high throughput screening.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE