Screening and Profiling for Kinases: Development of a Unique and Versatile Platform
Poster Nov 10, 2005
M. Chambon, A. Otto-Bruc, V. Hamon, C. Pernelle – Cerep, BP1, Le Bois L’Evêque, 86600 Celle L’Evescault, France
To build a unique kinase platform providing reliable and quantitative data, we have designed a flexible system with well-defined yet different assay conditions for each kinase. This platform minimizes interferences, including those from test compounds, and displays a homogeneous and generic assay format.
After reviewing available assay technologies, Homogeneous Time Resolved Fluorescence (HTRF) was selected as that which best met our requirements. To date, 70 kinase assays have been developed on this model. Using pertinent examples, we will illustrate how this technology supports and serves scientific drug discovery, and allows direct and obvious comparison across the panel for both high throughput compound profiling and high throughput screening.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE