Screening for Optimal Algal Cell Growth and Neutral Lipid Production Conditions in Microplates
Poster Feb 15, 2012
Paul Held, Xavier Amouretti, Wayne Patton and Peter Banks
This posters describes the quantitation of algal cell growth under various growth media conditions using a microplate reader to make absorbance and fluorescent determinations. Light scatter absorbance measurements at 600 nm were used to monitor cell growth and Nile Red stain fluorescence monitored neutral lipid production under various nutrient deficient states. Algal cells grow to a 20-fold greater density in complete nutrient rich media than in either nutrient poor or nitrogen deficient media. However, despite the marked disparity in cell number, cultures grown in nitrogen deficient media exhibit more neutral lipid staining. On a per cell basis Chlorella vulgaris cultures grown in nitrogen deficient media have 15-fold more lipid than cells grown in complete media. When nutrient deprived cells are placed in complete media, lipid droplets are quickly reabsorbed and Nile red staining returns to basal levels. The use of microplates enables the rapid measurement of multiple samples and experimental conditions at the same time.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE