Screening Medical Marijuana for Pesticides by GC-MS/MS
Di Wang, Laura Chambers, William Lipps, Zhuangzhi ‘Max’ Wang, Robert Clifford; Shimadzu Scientific Instruments, Columbia, MD, USA
Since medical marijuana (MM) was legalized in California in 1996, 23 states and Washington, D.C. have passed laws allowing its use for a variety of medical conditions. From a consumer safety point-of-view, quantitation of the pesticide residues in MM products has begun to attract wide interest. There are several problems associated with analysis of pesticide residues in MM. First and foremost, there are very few regulatory guidelines established to define which pesticides to include or what the detection limits should be, and secondly the matrix is very complex with significant interferences. Finally, sample load is growing exponentially, so the chosen method must be quick and easy to perform. Trace level pesticide analysis in complex food matrices have been done for many years with similar challenges, thus many of the analytical protocols emerging for the MM matrix are based on these well-established techniques.
Triple-quadrupole GC-MS/MS operated in MRM mode provides significant sensitivity and selectivity, but method development can be expensive and time consuming. This poster describes streamlined method development process for analysis of pesticide residues in MM using a QuEChERS sample preparation method, followed by GC-MS/MS detection and quantitation.
Proteomics and Substrate Based MS Imaging of Xenobiotic Metabolising Enzymes in Ex Vivo Human Skin and a Human Living Skin Equivalent ModelPoster
Untargeted proteomics analysis showed that human skin and a commercially available living skin equivalent model exhibit a similar distribution of xenobiotic metabolising enzymes. A new technique, substrate based mass spectrometry imaging (SB-MSI) was developed during this study.READ MORE
Development and Assessment of Non-Infected and Infected Skin Models Using MALDI-MSIPoster
Infections have detrimental effects on wound healing leading to chronic wound formation. The use of labskin,a living skin model, in combination with mass spectrometry imaging, provides a good model for assessing wound healing factors.READ MORE
Overcoming the Challenges of Analysing Ionic Polar Pesticides in FoodPoster
Here, the novel application of the Waters’ Torus DEA column is presented, showing the improved performance of a UPLC-MS/MS method for the underivatised
analysis of glyphosate and a selection of other anionic pesticides.