Screening Solution: ActivityBase™
Poster Feb 01, 2007
Jonathan Davies and Andrew English
The data management needs of discovery biology are complex and varied. The experimental data that is captured, stored and analyzed ranges from high volume, routine screening data through to low throughput, non-routine test management data that underpins pre-clinical research. ActivityBase™ is a data management system that integrates research data in a structured and scalable environment, providing secure management of a discovery organization’s principle asset – data.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE