Searching for Urinary Biomarkers of Exposure to Heterocyclic Amines (HCAs)
Poster Sep 09, 2014
Rosa Busquets, Henrik L. Frandsen, Kerstin Skog
Searching for urinary biomarkers of exposure to heterocyclic amines
R. Busquets1*, H. Frandsen2, K. Skog3.
1School of Pharmacy and Chemistry, Kingston University, Penrhyn Road, Kingston Upon Thames, Surrey, KT1 2EE.
2National Food Institute, Technical University of Denmark, DK-2860, Søborg, Denmark
3Department of Food Technology, Engineering and Nutrition, Lund University PO Box 124, SE-221-00 Lund, Sweden.
The Maillard reaction takes place in cooking processes and yields a wide range of compounds. Heterocyclic amines (HCAs) are mutagenic products that form in the Maillard when cooking meat or fish [1,2]. The formation of HCAs depends on the cooking conditions, such as temperature and time, and presence of chemical precursors or inhibitors. With the aim to find biomarkers of exposure to HCAs in accessible tissue, metabolites from HCAs have been determined in the urine of a group of 8 volunteers who ate a controlled meal consisting of fried chicken cooked for 6 min at 180 °C. The controlled meal accounted for a total of 6 μg of HCAs. The urine was analysed by a method based on liquid-phase microextraction and liquid chromatography-tandem mass spectrometry developed by our group , able to detect HCAs at concentrations as low as 2 pg/ ml urine. A narrow variation of one of the HCAs, 2-amino-6-phenylimidazo[4,5-b]pyridine in urine was observed among the volunteers: 0.91-2.1% of the initial dose from the meal, which let us propose this HCA as potential biomarker . Furthermore, new HCA-related metabolites have been identified in the urine samples. These finding will help to establish a relation between HCAs and cancer.
Profiling Personal Air Pollutant Exposures Using a Wearable Non-Selective Passive WristbandPoster
Characterizing cumulative exposure to air pollutant mixtures is a critical step in understanding disease development. The Fresh Air wristband was tested as a personal exposure assessment tool with promising results.READ MORE
Sport Doping Screening in Biological Matrices by Multi-Dimensional LC-QToFPoster
This work evaluated the performance of 2D LC variant using a QToF setup instead of a triple quadrupole mass spectrometer for the analysis of drug of abuse in urine targeting low and sub ppb level.READ MORE
Analysis of Doping and Forensic Drugs in Urine Using High-Resolution GC/Q-TOFPoster
In this study, we are examining the potential for high resolution accurate mass 7250 GC/Q-TOF equipped with low energy EI source, for both quantitative and screening aspects of doping control and forensic drugs applications.READ MORE