Simultaneous RT-qPCR Measurement of 1718 Long Non-Coding RNAs
Poster Sep 13, 2012
Pieter Mestdagh, Barbara D’haene, Jan Hellemans and Jo Vandesompele
Currently, expression and function of lncRNAs during human disease and development is largely unexplored. Few platforms are available that allow sensitive and specific high-throughput lncRNA expression profiling.
Study concludes, among other things, that lncRNA expression profiling of 60 cancer cell lines representing 9 different tumour entities reveals cancer-specific lncRNA expression profiles.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE