Single Cell Whole Genome Amplification
Poster May 21, 2015
The project presented here builds on the merits of conventional MDA to develop methods to recover and amplify DNA from single cells of bacterial and mammalian origin and discusses the methods & results of the new formulation. New optimized formulation increases the amplification sensitivity down to 1fg amount of DNA. Single cell whole genome amplification, SNP arrays and the NGS results suggest that the Single Cell GenomiPhi kit provides improved sensitivity and coverage with reduced amplification bias.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE