Single Cell Whole Genome Amplification
Poster May 21, 2015
The project presented here builds on the merits of conventional MDA to develop methods to recover and amplify DNA from single cells of bacterial and mammalian origin and discusses the methods & results of the new formulation. New optimized formulation increases the amplification sensitivity down to 1fg amount of DNA. Single cell whole genome amplification, SNP arrays and the NGS results suggest that the Single Cell GenomiPhi kit provides improved sensitivity and coverage with reduced amplification bias.
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s DiseasePoster
Here we evaluate the ability to detect changes in phosphorylation levels of ERK and CREB following treatment with Aβ using the SH-SY5Y neuroblastoma cell line.READ MORE
Characterizing GPCR Activation Using Automated Live Cell ImagingPoster
Here we describe a live cell imaging based approach to detect GPCR activation using the Lionheart™ FX Automated Live Cell Imager and Gen5™ Microplate Reader and Imager Software.READ MORE