Single Cell Whole Genome Amplification
Poster May 21, 2015
The project presented here builds on the merits of conventional MDA to develop methods to recover and amplify DNA from single cells of bacterial and mammalian origin and discusses the methods & results of the new formulation. New optimized formulation increases the amplification sensitivity down to 1fg amount of DNA. Single cell whole genome amplification, SNP arrays and the NGS results suggest that the Single Cell GenomiPhi kit provides improved sensitivity and coverage with reduced amplification bias.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE