Single Cell Whole Genome Amplification
Poster May 21, 2015
The project presented here builds on the merits of conventional MDA to develop methods to recover and amplify DNA from single cells of bacterial and mammalian origin and discusses the methods & results of the new formulation. New optimized formulation increases the amplification sensitivity down to 1fg amount of DNA. Single cell whole genome amplification, SNP arrays and the NGS results suggest that the Single Cell GenomiPhi kit provides improved sensitivity and coverage with reduced amplification bias.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE