Single Cell Whole Genome Amplification

The project presented here builds on the merits of conventional MDA to develop methods to recover and amplify DNA from single cells of bacterial and mammalian origin and discusses the methods & results of the new formulation. New optimized formulation increases the amplification sensitivity down to 1fg amount of DNA. Single cell whole genome amplification, SNP arrays and the NGS results suggest that the Single Cell GenomiPhi kit provides improved sensitivity and coverage with reduced amplification bias.