Specificity and Functionality of microRNA Inhibitors
Poster Mar 10, 2015
Barbara Robertson, Andrew Dalby, Jon Karpilow, Anastasia Khvorova, Devin Leake and Annaleen Vermeulen
Highly potent microRNA (miRNA) inhibitors are valuable tools for elucidating the roles of miRNAs and their targets. Although it is known that pairings between endogenous miRNAs and their natural targets in animals generally involve base pair mismatches, studies of how mismatches between endogenous miRNAs and artificial inhibitor targets might affect inhibitor specificity and functionality have been very limited. Using a Luciferase reporter system we have investigated the specicity of miRNA inhibitors. We first confirmed significant levels of cross-reactivity among the closely related let-7 family members. Subsequently,
a systematic study of mismatches incorporated into inhibitors of single-family-member miRNAs identified two regions that are important for overall inhibitor functionality. Our findings indicate that features important for natural miRNA target recognition also appear to be important for inhibitor specificity. Understanding the specificity of inhibitors allows for better interpretation of inhibitor activity in endogenous systems.
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
A new method for generating arrayed RNAi screening tools for any organismPoster
RNA interference (RNAi) using small interfering RNAs (siRNAs) is an important technology for down-regulation of gene expression and a powerful tool to study cellular processes and pathways. Previously, large collections of siRNAs were available only for traditional experimental model systems, such as human and mouse, and predominantly provided as chemically synthesized libraries.READ MORE
MicroRNAs as Bile-Based Biomarkers in Pancreatic Cancer and Biliary Tract CancersPoster
In this study, the role of miRNAs has been assessed in a large cohort of pancreatic cancer.READ MORE