Specificity of Highly Potent miRNA Inhibitors
Poster Feb 06, 2015
Barbara Roberston, Andrew Dalby, Yuriy Fedorov, Jon Karpilow, Anastasia Khvorova, Devin Leake, Annaleen Vermeulen
Highly potent miRNA inhibitors represent invaluable tools for elucidating the roles of miRNAs. However, it is important to be aware that such potent inhibitors may also affect function of other miRNAs, especially miRNA family members. To understand the potential cross-reactivity of miRNA inhibitors, various miRNA inhibitor designs were systematically tested. We demonstrate that mismatches both within and outside the seed region of the miRNA interfere with inhibition. Our findings indicate that certain features important for natural miRNA target recognition also appear to be important for inhibitor specificity.
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
A new method for generating arrayed RNAi screening tools for any organismPoster
RNA interference (RNAi) using small interfering RNAs (siRNAs) is an important technology for down-regulation of gene expression and a powerful tool to study cellular processes and pathways. Previously, large collections of siRNAs were available only for traditional experimental model systems, such as human and mouse, and predominantly provided as chemically synthesized libraries.READ MORE
Nitrogen metabolism in Mycobacterium tuberculosis: a systems-based approachPoster
Previous studies suggested that Mycobacterium tuberculosis obtains nitrogen from a diverse range of intracellular nutrients including amino acids. Here, we use a novel system’s based three-pronged approach to define pathways for uptake and assimilation of nitrogen.READ MORE