SPR Imaging of Protein Microarrays for Detection of Antibody Binding
Poster Feb 01, 2007
J.B. Beusink, G.A.J. Besselink and R.B.M. Schasfoort
Surface Plasmon Resonance imaging (SPR imaging) is an optical technique that, in combination with microarrays, can be used to study the kinetics of multiple biomolecular interactions. The main advantage is the ability to measure in a label-free and real-time manner.
In our study we have used the imaging SPR Instrument for Biomolecular Interaction Sensing (iSPR-IBIS) (IBIS Technologies BV, Hengelo, The Netherlands), for monitoring the binding of biomolecules to a protein array in a fully automated manner. A TopSpot instrument from BioFluidix GmbH (Freiburg, Germany) was used to spot the protein array.
By using fluorescently labelled antibodies, the binding to the immobilized proteins monitored with SPR imaging, could be confirmed on the very same sensor with fluorescence microscopy.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE