SPR Imaging of Protein Microarrays for Detection of Antibody Binding
Poster Feb 01, 2007
J.B. Beusink, G.A.J. Besselink and R.B.M. Schasfoort
Surface Plasmon Resonance imaging (SPR imaging) is an optical technique that, in combination with microarrays, can be used to study the kinetics of multiple biomolecular interactions. The main advantage is the ability to measure in a label-free and real-time manner.
In our study we have used the imaging SPR Instrument for Biomolecular Interaction Sensing (iSPR-IBIS) (IBIS Technologies BV, Hengelo, The Netherlands), for monitoring the binding of biomolecules to a protein array in a fully automated manner. A TopSpot instrument from BioFluidix GmbH (Freiburg, Germany) was used to spot the protein array.
By using fluorescently labelled antibodies, the binding to the immobilized proteins monitored with SPR imaging, could be confirmed on the very same sensor with fluorescence microscopy.
Applications of chemically modified synthetic guide RNA for CRISPR-Cas9 genome editingPoster
Our results indicate that MS modifications are required for experiments with co-electroporation of Cas9 mRNA and synthetic gRNA, yet have no impact on editing efficiency when delivered with lipid-based transfection reagents.READ MORE
Functional Analysis of RAD51B and RAD23B SNPs using Circular Chromosome Conformation Capture (4C) in Human Prostate Cell LinesPoster
The aim of this study is to understand the mechanism behind the association between single nucleotide polymorphisms (SNPs), lying in non-coding regions near or within RAD51B and RAD23B, and prostate cancer risk by analysing their interactions with the whole genome using Circular Chromosome Conformation Capture (4C).READ MORE
Preparation of Photonic Biosensors by Inkjet Printing TechnologyPoster
In order to enable local functionalization of label-free optical waveguide biosensors in a cost effective mass-fabrication compatible manner, we investigate surface modification employing inkjet printing of functional polymers and UV-curable benzophenone dextran.READ MORE