SPR Imaging of Protein Microarrays for Detection of Antibody Binding
Poster Feb 01, 2007
J.B. Beusink, G.A.J. Besselink and R.B.M. Schasfoort
Surface Plasmon Resonance imaging (SPR imaging) is an optical technique that, in combination with microarrays, can be used to study the kinetics of multiple biomolecular interactions. The main advantage is the ability to measure in a label-free and real-time manner.
In our study we have used the imaging SPR Instrument for Biomolecular Interaction Sensing (iSPR-IBIS) (IBIS Technologies BV, Hengelo, The Netherlands), for monitoring the binding of biomolecules to a protein array in a fully automated manner. A TopSpot instrument from BioFluidix GmbH (Freiburg, Germany) was used to spot the protein array.
By using fluorescently labelled antibodies, the binding to the immobilized proteins monitored with SPR imaging, could be confirmed on the very same sensor with fluorescence microscopy.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE