Statistical Significance of Proteome Analysis
Poster Apr 05, 2007
Stefan Muellner, Andreas Wattenberg, Katja Aschermann and Petra Lutter
Standard Operation Procedures for each and every step within the work flow of proteome analysis is key for the statistical relevance of the data obtained. We have compared the data sets of four larger proteome studies of different biological samples with regards to the influence of the major steps - sample preparation, protein quantification, study design, analysis, and statistics - on the coefficient of validation of the whole process.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE