Study Of The Active Bacterial Community In Two Membrane Bioreactors
Poster May 06, 2015
Moreno-Mesonero, L.1, Moreno, Y.1*, Morillo, J.A., Muñagorri, F.2 and Alonso, J.L.1
Membrane bioreactor (MBR) systems allow for the separation of mixed liquor and treated water thanks to membranes. Bacterial communities in MBRs can be quite different from conventional ones due to the long retention time and, therefore, the low metabolic cellular activity. Moreover, MBRs have better treated water quality, are able to operate with high biomass concentrations and their effluent is of great quality and can be recirculated. An extensive research on the bacterial communities in these systems is essential to achieve a good treatment performance.
In this work, bacterial communities from two MBR systems treating leachates were evaluated using the 16S rRNA metagenomics approach, with and without a viability dye (Propidium Monoazide, PMA).
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Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE