Study Of The Active Bacterial Community In Two Membrane Bioreactors
Poster May 06, 2015
Moreno-Mesonero, L.1, Moreno, Y.1*, Morillo, J.A., Muñagorri, F.2 and Alonso, J.L.1
Membrane bioreactor (MBR) systems allow for the separation of mixed liquor and treated water thanks to membranes. Bacterial communities in MBRs can be quite different from conventional ones due to the long retention time and, therefore, the low metabolic cellular activity. Moreover, MBRs have better treated water quality, are able to operate with high biomass concentrations and their effluent is of great quality and can be recirculated. An extensive research on the bacterial communities in these systems is essential to achieve a good treatment performance.
In this work, bacterial communities from two MBR systems treating leachates were evaluated using the 16S rRNA metagenomics approach, with and without a viability dye (Propidium Monoazide, PMA).
The novel ‘K’ DNA extraction protocol offers a reliable alternative for preparing fecal specimens for 16S rRNA gene amplicon sequencing that maintains representation of microbial populations in a sample, with the added benefits that the K method reduces hands-on time by up to 20 x for 96 sample preparations.READ MORE