We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
T7 Amplification for Generating Single Stranded Target for Hybridization to Allele-Specific DNA Microarrays
Poster

T7 Amplification for Generating Single Stranded Target for Hybridization to Allele-Specific DNA Microarrays

T7 Amplification for Generating Single Stranded Target for Hybridization to Allele-Specific DNA Microarrays
Poster

T7 Amplification for Generating Single Stranded Target for Hybridization to Allele-Specific DNA Microarrays

Abstract
Amplified RNA (aRNA) synthesized by T7 linear amplification is frequently used for gene expression analysis on microarrays. Single stranded DNA (ssDNA) is however mostly used as target in microarray based genotyping assays, although there are several advantages with aRNA. Here, we compared the performance of aRNA and ssDNA as targets in a genotyping assay, comprising a DNA microarray of allele specific oligo probes specific for thalassemic mutations.
Advertisement