T7 Amplification for Generating Single Stranded Target for Hybridization to Allele-Specific DNA Microarrays
Abstract
Amplified RNA (aRNA) synthesized by T7 linear amplification is frequently used for gene expression analysis on microarrays. Single stranded DNA (ssDNA) is however mostly used as target in microarray based genotyping assays, although there are several advantages with aRNA. Here, we compared the performance of aRNA and ssDNA as targets in a genotyping assay, comprising a DNA microarray of allele specific oligo probes specific for thalassemic mutations.
Amplified RNA (aRNA) synthesized by T7 linear amplification is frequently used for gene expression analysis on microarrays. Single stranded DNA (ssDNA) is however mostly used as target in microarray based genotyping assays, although there are several advantages with aRNA. Here, we compared the performance of aRNA and ssDNA as targets in a genotyping assay, comprising a DNA microarray of allele specific oligo probes specific for thalassemic mutations.
