Temporal Dynamics of Pathogenesis Related Metabolites and their Metabolic Pathways Following Inoculation of Potato Leaves with Phytophthora infestans
Poster Mar 09, 2007
Y. Abu-Nada, A. C. Kushalappa, W. D. Marshall, K. Al-Mughrabi, and A. Murphy
The resistance in potato cultivars against late blight caused by Phytophthora infestans is classified into vertical and horizontal. Several metabolites associated with resistance have been detected: phytoalexins such as rishitin, phytuberin, lubimin, solavetivone etc. Metabolite profiling is a novel technology for the discrimination of quantitative resistance in plants against pathogen stress. Metabolite profiles based on GC/MS analysis have been used to discriminate resistance in wheat against Fusarium graminearum.
We developed an automated sample preparation protocol based on a robotic platform PAL RTC (CTC Analytics AG, Zwingen Switzerland), which represent a modified Bligh and Dyer method producing samples for both hydrophilic metabolomics using GC-MS and lipidomics using SFC-MS simultaneously.READ MORE