Testing a Novel Real Time Cell Viability Assay
Poster Oct 09, 2015
Amy Landreman, Sarah Duellman, Wenhui Zhou, Jolanta Vidugiriene, Brad Hook
Recently developed assay technologies make it possible to use multi-well plate readers to measure the number of live or dead cells in culture in real time over a period of days. Live cells are measured in real time by adding a reagent containing a shrimp-derived luciferase and a pro-substrate directly to the culture medium. Only viable cells can convert the pro-substrate into a luciferase substrate and generate light. The real time viability assay is non-toxic to cells, so viable cells remain in the sample well following measurement of the live cell signal. In addition to providing real time kinetic measurements that are valuable for assay development and characterization activities, multiplexing with other assays (e.g. dead cell staining, apoptosis, oxidative stress markers, reporter gene assays or RNA extraction) provides a time saving approach and statistical advantage inherent in taking measurements from the same sample of cells.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE