THe AtSCL26 Transcription Factor Controls Cross-talk Between GA and N Root Architecture in Arabidopsis Thaliana Roots
Poster Apr 14, 2015
Beatriz Lagunas, Anthony D. Carter, Dafyd Jenkins and Miriam Gifford
Multicellular organisms such as plants rely on highly specialised tissue-specific cell-types to perform discrete functions, and this specialisation enables modulation of plant productivity. Cell-type identity is also involved in determining which cells are capable of sensing internal and external conditions and the amplitude of downstream responses. In roots, aside from mediating growth and nutrient uptake, cell-speciﬁc transcriptional programs are fundamental to the developmental organisation of root architecture including formation of lateral branching roots that enable the root to harvest nutrients, and root nodule structures. Root nodules form to house symbiosis between legume species with soil nitrogen fixing bacteria (rhizobia). Phenotypic and molecular evidence supports the hypothesis that developmental program enabling nodule formation arose during evolution 70 MYA from a lateral root ‘blueprint’ pre-existing in all higher plants .
Understanding the conserved regulation of development has high relevance for building resilience in food security and agriculture. Molecular integrators of environmental controlling development could act across multiple pathways, thus we reasoned that analyzing Arabidopsis genes orthologous to regulators of nodulation could shed insight on control of lateral root development. This led us to the discovery that an Arabidopsis GRAS family transcription factor controls lateral root development under specific nitrogen conditions. Since AtSCL26 is the putative ortholog of the key nodulation regulator MtNSP2 in Medicago truncatula, this gene appears to be a conserved developmental regulator. We also identified effects of AtSCL26 that are specific to epidermal and cortical cells using Fluorescence Activated Cell Sorting (FACS), paralleling the cell type specific effects found for the nitrogen-regulated MtNSP2.
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.