The BioRobot® Universal System - Multiple Applications on one Instrument
Poster Apr 12, 2006
Thomas Weierstall, Anja Schultz, Daniel Langendörfer, Martin Heller and Carola Schade
AbstractThe BioRobot Universal System enables standardized sample preparation and reaction setup using optimized, ready-to-run protocols. Fully tested protocols provide purification of RNA from blood, cells, and tissue and purification of genomic DNA from blood and buccal swabs, as well as RT-PCR and PCR setup. Users can easily switch between applications without the need for hardware configuration changes. This study was conducted to show the performance of the BioRobot Universal System in gene expression and genotyping applications.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE