The Comparison of Lipid Extraction Methods for the Lipidomic Analysis of Human Blood Plasma
Poster Jan 24, 2007
Although metabolomics is and should remain an integrated approach by itself, its complexity requires analogous approaches focused on its components, such as lipidomics. Lipid profiling by NMR without sample extraction, should be seen as only the first stage in lipidomic analysis. Subsequent analysis should involve extraction of the lipids from the biofluid or tissue prior to further profiling using a multi-technique metabolomic approach involving both NMR and MS.
We developed an automated sample preparation protocol based on a robotic platform PAL RTC (CTC Analytics AG, Zwingen Switzerland), which represent a modified Bligh and Dyer method producing samples for both hydrophilic metabolomics using GC-MS and lipidomics using SFC-MS simultaneously.READ MORE