The Future of Compound Management
Poster Jul 05, 2011
Dr Richard Kim, Ben Schenker, Simon Tullett
Current compound management practices have evolved to support both primary and secondary screening projects from a centralized repository storing a combination of plates and tubes. In this poster we will describe an innovative large-scale tube-based compound management approach to maximize lab space; improve compound stability; enable rapid generation of custom screening sets; while providing background QC and real time library integration of new chemical entities.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE