The Role of microRNAs in Memory Consolidation in Lymnaea
Poster Nov 01, 2015
György Kemenes1, Dimitris Vavoulis2, Sergei Korneev1
After single-trial classical conditioning there are well-defined time windows of activation of and requirement for key ‘conventional’ molecular players in the different phases of the consolidation of longterm memory (LTM) in Lymnaea. Two important related discoveries we have made recently are:
i) late LTM (24h post-training) requires transcription at 6h post-training
ii) at 6h post-training, there is ongoing phosphorylation of CREB1 and increased acetylation of H3, both of which can be measured in the ‘learning ganglia’ as well as in single identified neurons known to be involved in learning
However, the requirement for new protein synthesis for LTM only lasts for up to 1h after conditioning. Together, these findings gave rise to the hypothesis that newly transcribed non-coding RNAs (e.g., miRNAs) are involved in the early as well as intermediate-term phase of memory consolidation. We tested this hypothesis by investigating the temporal dynamics of the post-training expression of miRNAs in the ‘learning ganglia’ of Lymnaea.
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.