The Synergy™ 2, A Novel Approach to Microplate Multi- Detection for HTS and Drug Discovery
Poster Feb 01, 2007
Paul Held and Xavier Amouretti
The Synergy™ 2 is a new type of reader that provides research laboratories performances usually found on high-end HTS instrumentation, while delivering flexibility and efficient cost-control to screening laboratories. The Synergy 2 utilizes multiple optics to provide performance regardless of the detection technology. Absorbance measurements use a xenon lamp with a monochromator for wavelength selection from 200 nm to 999 nm. Fluorescence measurements are made using either a tungsten-halogen or a xenon-flash lamp with filters and dichroic mirrors and a PMT for detection.
Fluorescence polarization is accomplished with polarizing filters in conjunction with dichroic mirrors. For time-resolved fluorescence measurements, the Synergy 2 integrates a high-energy xenon-flash lamp with excitation and emission filters and PMT detector. Luminescence measurements are accomplished by using a liquid-filled light guide. The Synergy 2 is capable of reading any plate format up to 1536 wells, is robotic compatible and provides temperature control and shaking. Reagent injectors are also available.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE