The Use of Software Visualization Tools for the Identification of PTM Patterns and Artefacts in LC-MS Experiments
Poster Aug 25, 2005
Harald Pettersen Lennart Björkesten and Matthias Berg
Traditional analysis of LC-MS data by reviewing chromatograms and corresponding single or averaged mass spectra is evidently both tedious and difficult. When analyzing multiple proteomics samples, this part of the data analysis is therefore often omitted and only the final list of identified proteins is reviewed. This may lead to unnecessarily complex or even contradictory results.
By representing LC-MS data as a 2D-image as demonstrated in the novel DeCyder™ MS software, it is possible to identify phenomena, which are very difficult to recognize using conventional LC-MS data representation. By discarding appropriate phenomena as artefacts the quality of the result will be significantly improved.
Identifying Non-Intentionally Added Substances (NIAS) in Food Contact MaterialsPoster
Concern about the safety of food products has increased dramatically with intentionally and non-intentionally added substances (NIAS) used in packaging being of particular interest. APGC and high resolution MS together with a workflow driven process was used to identify unknown compounds in packaging.READ MORE
Human exposure to cyanotoxins: Exploring in vitro detoxification using atmospheric cold plasma treatment to protect human healthPoster
Recently, there have been increases in freshwater harmful algal blooms. There is therefore a need to try and effectively detoxify drinking water contaminated with cyanotoxins to safeguard human health by use of new and novel techniques.READ MORE
C1 Complement mediates human cord blood serum derived APP α-secretase cleavage activity in vitroPoster
Our results indicate that CBS contains proteins that promote α-secretase like enzymatic activity. LC-MS/MS analysis in CBSF and AgBSF revealed the presence of 142 proteins of which C1 subunits and alpha-2-macroglobulin showed significantly greater levels in αCBSF compared with αAgBSF. further study showed,C1 subunits can enhance sAPPα production and Aβ reduction in cell culture conditionREAD MORE