Three-Dimensional Cell Culture Within A Biomimetic Hydrogel Based On Chitosan And Hyaluronan
A growing demand for study of cells within biologically realistic, in vitro, microenvironments requires a paradigm shift in cell culture technology: from cell culture on 2-D, rigid substrata to cell growth within a 3-D space, defined by malleable materials composed of biologically “smart” compounds.1, 2
Therapeutic issues addressed by tissue engineering and regenerative medicine require similar materials, customized for human application in a wide variety of treatment circumstances: ranging from the ideal operating room to battle field conditions.