TMT Quantification for Shotgun Proteomics: TIMS-QTOF Powered by PASEF
Poster May 31, 2019
Matt Willetts, Shourjo Ghose, Heiner Koch, Scarlet Koch, Markus Lubeck, Gary Kruppa.
This poster evaluates the performance of ‘timsTOF Pro’ a unique dual trapped ion mobility spectrometer (TIMS) coupled to a QTOF for the analysis of isobaric labelling experiments.
Download to discover:
- How TIMS provides an additional dimension of separation and high peak capacity
- Why the timsTOF Pro instrument is well suited to isobaric labelled quantitation experiments
- How to achieve near 100% duty cycle for shotgun proteomics
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.
Like what you just read? You can find similar content on the communities below.Analysis & Separations Applied Sciences Biopharma Cell Science Diagnostics Drug Discovery Informatics Proteomics & Metabolomics
To personalize the content you see on Technology Networks homepage, Log In or Subscribe for FreeLOGIN SUBSCRIBE FOR FREE