Transcription Factors to Classify Tumor Types and Subtypes
Poster Dec 12, 2014
Benjamin Otto 1,2, Kristin Klätschke 2, Thomas Streichert 3, Christoph Wagener 2, Genrich Tolstonog 4
Awareness of the role in tumor biology of epigenetics and transcription regulatory mechanisms, such as miRNAs, is increasing. We assume that transcription factors play a crucial role in that context, such that they might be capable of discriminating different tumor classes and yet unknown subgroups. Our approach aims at unsupervised identification of a) sample subsets within a dataset and b) the TFs associated with these subsets. The results show, that an unsupervised selection of TFs can clearly distinguish between the classic breast cancer subtypes and different tumor types such as breast, colon, kidney or prostata cancer. Therefore we assume that the method might help in future to identify new markers suitable for diagnosis of specific treatment related phenotypes or detection of tissues of origin.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE