Transfecting Small Molecules, Peptides
Poster Nov 19, 2010
Van Q., Atze K., Litzenberger D., Ackerstaff J.T., Strübing Y., Yolcu D., Franke S.,Mobbs K.J. and Kazinski M.
The Nucleofector® Technology enables efficient and reproducible transfection of primary cells and cell lines at throughputs of up to 96 samples per run.
Nucleofection® now extends its range of application to deliver small molecules substrates and protein substrates such as peptides, proteins and antibodyconjugates.
The Nucleofector 96-well Shuttle® System combines the proven advantages of Nucleofection with the flexible throughput capacity to enable cell-based screening approaches using RNAi or peptide libraries, early ADME-Tox applications using small molecules, or pathway validation using inhibitory peptides or antibodies.
The delivery of small molecules, peptides and antibodies is presented, demonstrating the capabilities of the Nucleofector Technology and its potential to enhance standard applications in pharmaceutical research and drug discovery.
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
RNA interference (RNAi) using small interfering RNAs (siRNAs) is an important technology for down-regulation of gene expression and a powerful tool to study cellular processes and pathways. Previously, large collections of siRNAs were available only for traditional experimental model systems, such as human and mouse, and predominantly provided as chemically synthesized libraries.READ MORE