Transfection Efficiency and Cytotoxicity of Commonly Used siRNA Transfection Reagents
Poster Jan 18, 2007
Maria Majorek, Monika Lamparska-Przybysz, Piotr Guzenda and Maciej Wieczorek
The present study was focused on the comparison of efficiency and cytotoxicity of five most popular transfection reagents: Lipofectamine 2000, Lipofectamine RNAi MAX (Invitrogen), Dharmafect (Dharmacon), HyperFect (Qiagen) and Interferin (PolyTransfection). In this study we observed that except Lipofectamine RNAi MAX, other reagents have relatively high cytotoxicity and low transfection efficiency in MCF-7 and HeLa cells.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.