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Transfection Efficiency and Cytotoxicity of Commonly Used siRNA Transfection Reagents

The present study was focused on the comparison of efficiency and cytotoxicity of five most popular transfection reagents: Lipofectamine 2000, Lipofectamine RNAi MAX (Invitrogen), Dharmafect (Dharmacon), HyperFect (Qiagen) and Interferin (PolyTransfection). In this study we observed that except Lipofectamine RNAi MAX, other reagents have relatively high cytotoxicity and low transfection efficiency in MCF-7 and HeLa cells.