Two secreted orthogonal bioluminescent reporters for non-destructive ultrasensitive assays
Poster May 11, 2010
George Tzertzinis1, Salvatore Russello1, Cathy Olsen2
Here we present a powerful combination of two secreted bioluminescent reporters, the luciferases from Gaussia princeps and Cypridina noctiluca. We demonstrate that the properties of these two reporters in terms of sensitivity, compatibility and use in non- destructive assays conducted on live cells or supernatants from different well formats makes them suitable for novel high throughput format applications.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License
OTHER POSTERS
Knockout of microRNAs Using the CRISPR-Cas9 System with Paired Synthetic crRNAs
PosterWe utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.
READ MORE
Exploiting Polypharmacology in Precision Oncology: Identification of Differential Kinase Off-targets Among Clinical PARP Inhibitors
PosterCan we use computational methods to identify previously unknown off-targets of PARP inhibitors that can explain their observed differences?
READ MORE
Bioluminescent Assay for GTPases Allows Measurement of GTPase, GAP and GEF Activities
PosterWe have developed a homogenous bioluminescent assay (GTPase-Glo) system to analyze these proteins in a simple, convenient “add-mix-read” format.
READ MORELike what you just read? You can find similar content on the communities below.
Drug DiscoveryTo personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free
LOGIN SUBSCRIBE FOR FREE
5th edition of the International Conference Clinical Oncology and Molecular Diagnostics
Jun 17 - Jun 18, 2019
