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Using the Promega GloSensor™ cAMP technology on the FLIPR® Tetra system for live cell Gi- and Gs- coupled GPCR second messenger assays

The poster also demonstrates endogenous receptor activity in CHO-K1 and HEK-293 cell lines stably expressing the GloSensor plasmid. In addition, transfected Gs- and Gi-coupled receptor activity are measured from cell lines with stably transfected GPCR receptors and transient transfection of the GloSensor plasmid. Multiplexing Gq- and Gs-coupled GPCR assays was shown to be possible using the GloSensor cAMP assay and FLIPR® Calcium 5 Kit in the same well. Combined with the GloSensor cAMP Assay, the FLIPR® Tetra system delivers the complete solution for kinetic screening of the major classes of GPCR subtypes.

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