Using the Promega GloSensor™ cAMP technology on the FLIPR® Tetra system for live cell Gi- and Gs- coupled GPCR second messenger assays
Poster Oct 05, 2011
J. Pschorr, S. Lydford, C. Crittenden and Y.-W. Chen
The poster also demonstrates endogenous receptor activity in CHO-K1 and HEK-293 cell lines stably expressing the GloSensor plasmid. In addition, transfected Gs- and Gi-coupled receptor activity are measured from cell lines with stably transfected GPCR receptors and transient transfection of the GloSensor plasmid. Multiplexing Gq- and Gs-coupled GPCR assays was shown to be possible using the GloSensor cAMP assay and FLIPR® Calcium 5 Kit in the same well. Combined with the GloSensor cAMP Assay, the FLIPR® Tetra system delivers the complete solution for kinetic screening of the major classes of GPCR subtypes.
CiPA Phase 2 Study: validation of an automated microelectrode array (MEA) assay of hiPSC-derived cardiomyocyte electrophysiology for cardiac safety evaluationPoster
These results support the use of hSC-CM and MEA technology for preclinical assessment of proarrhythmic risk within the proposed CiPA paradigm, and, more generally, demonstrate that automation of the CM-MEA assay can achieve high reliability and throughput for cardiac risk assessment in vitro.READ MORE
A luminescent solar concentrator-based photomicroreactor for energy efficient continuous-flow photocatalysisPoster
The Luminescent Solar ConcentratorPhotomicroreactor
(LSC-PM) is an innovative device
for solar-powered continuous-flow photochemistry.