Utility of 405nm-Excitable Dyes in High Content Screening Using an Acumen Explorer Microplate Cytometer
Abstract
The Acumen Explorer is a fluorescent microplate cytometer equipped with either a 405nm or 488 nm laser. The instrument offers rapid read and analysis times of individual cells within 96-3456 well plates (typically 5-10 minutes), making it compatible with the sustained use of high content methods in primary screens. In addition, small file sizes (>50kB in screening mode) are produced, therefore removing the requirement for expensive data storage solutions in compound screening programs.
The Acumen Explorer is a fluorescent microplate cytometer equipped with either a 405nm or 488 nm laser. The instrument offers rapid read and analysis times of individual cells within 96-3456 well plates (typically 5-10 minutes), making it compatible with the sustained use of high content methods in primary screens. In addition, small file sizes (>50kB in screening mode) are produced, therefore removing the requirement for expensive data storage solutions in compound screening programs.
In this study, we have used an Acumen Explorer equipped with a violet 405nm laser in conjunction with a selection of shorter-wavelength amine-reactive fluorophores (Invitrogen, Molecular Probes) to demonstrate a greater utility for blue fluorescent probes within high content assays.
