Validation of a Novel Tumoroid-Based Cell Culture Model to Perform 3D in vitro Cell Signaling Analyses
Poster Oct 08, 2014
Brad Larson 1, Grant Cameron 2, Nicolas Pierre 3, and Peter Banks 1
Here we demonstrate an in vitro HTRF® microplate assay that can quantify total, as well as phosphorylated elF4E. The assay used a novel 3D culture system called RAFT' to create cell accumulations termed tumoroids in a cell/collagen hydrogel mix. In addition, the assay was also performed with cells cultured using traditional 2D methods for comparison. Dispensing and removal steps were performed by the MultiFloTM FX Microplate Dispenser, including cell/collagen mix, medium, and reagent dispensing, as well as removal of spent medium and compounds. Detection of the fluorescent signals from the HTRF assay, as well as tumoroid imaging, was performed by the CytationTm 3 Cell Imaging Multi-Mode Reader. Validation data generated using RAFT confirms the robustness of the 3D system. Pharmacology comparison data from both methods also demonstrates the validity of using 3D cell culture for cell signaling analyses.
Extracorporeal shockwave therapy accelerates motor axon regeneration despite a phenotypically mismatched environmentPoster
A femoral nerve defect model was adapted for the evaluation of proregenerative effects of extracorporeal shockwave therapy (ESWT). Functional evaluation, histology and qRT-PCR data show differences between sensory and motor-derived nerve transplants and a pro-regenerative effect of ESWT. These data provide evidence for the clinical application of ESWT after autologous nerve transplantation as a novel non-invasive method.READ MORE
Histone Deacetylase 6 (HDAC6) As A Therapeutic Target in Chronic Lymphocytic LeukemiaPoster
This study investigates the role of histone deacetylase 6 (HDAC6) in chronic lymphocytic leukemia and establishes it as a novel therapeutic target for the treatment of this disease.READ MORE