Validation of an Automated Cell-Based Bioluminescent TNFa Blocker Bioassay

This poster demonstrates the automation of a 96-well homogeneous bioluminescent TNFα blocker bioassay based on quantification of caspase 3 activity. The bioassay can be performed in a single day, and uses single-use, frozen U937 (human) cells which exhibit rapid response to TNFα. A simple, yet robust liquid handler was used to automate the assay steps of antibody titration and of cell and reagent dispensing.