Validation of an Automated Cell-Based Bioluminescent TNFa Blocker Bioassay
Poster Feb 16, 2012
Brad Larson, Tracy Worzella, Rich Moravec, Neal Cosby, Frank Fan, Teresa Surowy and Peter Banks
This poster demonstrates the automation of a 96-well homogeneous bioluminescent TNFα blocker bioassay based on quantification of caspase 3 activity. The bioassay can be performed in a single day, and uses single-use, frozen U937 (human) cells which exhibit rapid response to TNFα. A simple, yet robust liquid handler was used to automate the assay steps of antibody titration and of cell and reagent dispensing.
Bioluminescent Assay for GTPases Allows Measurement of GTPase, GAP and GEF ActivitiesPoster
We have developed a homogenous bioluminescent assay (GTPase-Glo) system to analyze these proteins in a simple, convenient “add-mix-read” format.READ MORE
NanoBRET™ Assays for Monitoring Protein Interactions in Living CellsPoster
NanoBRET PPI System is composed of two components.
NanoBRET offers improved S:B over other BRET assays.
NanoBRET provides sensitive live-cell method to study protein interactions.
Human iPSC-derived cardiomyocytes: A translational model to predict drug-induced cardiac arrhythmias and long-term toxicityPoster
The results shown here imply that iPSC-derived Cor.4U human cardioymocytes are a translational in vitro cell model for the prediction of clinically relevant drug-induced cardiac arrhythmias and long-term toxicity.READ MORE