Validation of siRNAs - From Conceptual Design to Process
Poster Feb 01, 2007
Ute Krueger, Tobias Bergauer, Brigitte Kaufmann, Meike Isselhorst, Irene Wolter, Juliane Konrad, Mike Heider-Fabian,Steffi Kirch*, Christiane Artz-Oppitz, Meera Tiruvenkata, James Qin, Dong Liang, Subu Yerramilli, Eric Lader, and Jie Kang
To increase the chance of successful RNAi, we have designed and functionally tested a large number of siRNAs targeting important human genes and shown that they provide high knockdown efficiency. These HP Validated siRNAs allow researchers to avoid upfront design and testing experiments and to start experiments immediately.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE