Validation of siRNAs - From Conceptual Design to Process
Poster Feb 01, 2007
Ute Krueger, Tobias Bergauer, Brigitte Kaufmann, Meike Isselhorst, Irene Wolter, Juliane Konrad, Mike Heider-Fabian,Steffi Kirch*, Christiane Artz-Oppitz, Meera Tiruvenkata, James Qin, Dong Liang, Subu Yerramilli, Eric Lader, and Jie Kang
To increase the chance of successful RNAi, we have designed and functionally tested a large number of siRNAs targeting important human genes and shown that they provide high knockdown efficiency. These HP Validated siRNAs allow researchers to avoid upfront design and testing experiments and to start experiments immediately.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE