Why Is My Assay Failing? An Approach to Assay Equipment Optimization
Poster Feb 27, 2012
Tanya R. Knaide, John Thomas Bradshaw, Kevin Khovananth, Keith Albert
Some potential sources of error could include: 1) incorrect, contaminated or expired reagents, 2) technician error, 3) equipment error, etc. Even simple assays include multiple steps where errors could be introduced, which greatly increases the difficulty of finding the source of the error. Therefore, determining the cause of unexpected results commonly presents a time consuming and expensive task for laboratory researchers. One approach to reducing assay failure is to ensure equipment errors are removed or at least reduced. This may be accomplished by optimizing the equipment employed prior to running the assay. Traditionally, tools used to optimize the performance of this equipment have been varied, not standardized and not traceable. In this poster presentation, the use of the Artel MVS® Multichannel Verification System and PCS® Pipette Calibration System will be discussed for optimization of the laboratory equipment used at each step of any given assay including plate washers, plate mixers, large volume liquid dispensers, manual pipettes and automated liquid handlers.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE
During early drug discovery, the study of metabolism plays an essential role in determining which drug candidates move forward into development and later stages. As an alternative to traditional Data Dependent Acquisition (DDA), the use of MSE/All Ions Fragmentation (AIF) has become common in metabolite identification workflows for the analysis of metabolic hot spots. Here we present a solution for analysis of MSE/AlF in metID studies.READ MORE