Why Is My Assay Failing? An Approach to Assay Equipment Optimization
Poster Feb 27, 2012
Tanya R. Knaide, John Thomas Bradshaw, Kevin Khovananth, Keith Albert
Some potential sources of error could include: 1) incorrect, contaminated or expired reagents, 2) technician error, 3) equipment error, etc. Even simple assays include multiple steps where errors could be introduced, which greatly increases the difficulty of finding the source of the error. Therefore, determining the cause of unexpected results commonly presents a time consuming and expensive task for laboratory researchers. One approach to reducing assay failure is to ensure equipment errors are removed or at least reduced. This may be accomplished by optimizing the equipment employed prior to running the assay. Traditionally, tools used to optimize the performance of this equipment have been varied, not standardized and not traceable. In this poster presentation, the use of the Artel MVS® Multichannel Verification System and PCS® Pipette Calibration System will be discussed for optimization of the laboratory equipment used at each step of any given assay including plate washers, plate mixers, large volume liquid dispensers, manual pipettes and automated liquid handlers.
Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays for the 4200 TapeStation SystemPoster
Here, we focus on quantification, sizing, and sensitivity of both D5000 ScreenTape assays.READ MORE
CiPA Phase 2 Study: validation of an automated microelectrode array (MEA) assay of hiPSC-derived cardiomyocyte electrophysiology for cardiac safety evaluationPoster
These results support the use of hSC-CM and MEA technology for preclinical assessment of proarrhythmic risk within the proposed CiPA paradigm, and, more generally, demonstrate that automation of the CM-MEA assay can achieve high reliability and throughput for cardiac risk assessment in vitro.READ MORE