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Why Use Supported Liquid Extraction To Develop a GLP Protocol for the H295R Steroidogenesis Assay?

The Benefits of SLE in the Development of a GLP Protocol for the Measurement of 17β-Estradiol and Testosterone in the H295R Steroidogenesis Assay, Test No 456


Environmental chemicals have the potential to perturb hormone systems, a finding that has led to recommendations for the testing of potential endocrine disrupting chemicals. The Steroidogenesis H295R assay is an in vitro cell model used to investigate compound effects on steroid hormone biosynthesis, specifically 17β-estradiol and testosterone.


Download this poster to discover how using supported liquid extraction in the development of a robust GLP bioanalytical method:

  • Provides considerable cost savings both in terms of consumables and analyst time
  • Requires fewer resources
  • Is technically less challenging than liquid-liquid extraction
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