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AMS Biotechnology Releases Highly Reproducible Cell Migration Screening Assay
Product News

AMS Biotechnology Releases Highly Reproducible Cell Migration Screening Assay

AMS Biotechnology Releases Highly Reproducible Cell Migration Screening Assay
Product News

AMS Biotechnology Releases Highly Reproducible Cell Migration Screening Assay


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AMS Biotechnology has announced a new generation of novel two-dimensional cell-based assay kits developed for reproducibly screening test compounds that may stimulate or inhibit adherent cell migration.

The Oris™ Cell Migration Assay provides life scientists with a method that is less labor intensive, allows for real-time imaging, and is amenable to high throughput and high content imaging instruments.

Unlike traditional cell migration assays using a Boyden chamber device, there is no transmembrane or cell culture insert obstructing live images of the cells as they migrate.

Described in a new video the Oris™ Cell Migration Assay uses a multistep process that starts with the attachment (optional) of a detection mask to the bottom of the wells of the Oris™ 96-well assay microplate.

A specially designed, cell seeding stopper fitted inside of each microplate well restricts cells from seeding into a central detection zone as cells are being dispensed. After cell attachment has occurred (4-28 hrs), the stoppers are removed, allowing cells to freely migrate into the detection zone of the wells. Cells can be labeled pre- or post-migration.

Cells that have not migrated into the detection zone are blocked from view by the mask initially applied to the plate bottom. Migrated cells may then be analyzed using a fluorescence plate reader. If analyzing the migrated cells using a microscope or high content imaging system, the detection mask need not be attached.

Oris™ Cell Migration assays are available with tissue culture treated plates, Collagen I coated plates, Fibronectin coated plates, or a TriCoated version that contains all 3 surfaces.

Wound healing assays are normally performed using a scratch assay, which has high variability. The Oris™ Cell Migration Assay is an ideal alternative to the scratch assay since the cell seeding stoppers create a consistent detection zone to monitor 2-D closure allowing reproducible data to be collected.

The Oris Assay gives researchers the option to take real time, video images of cell movement or to quantify cell migration using a fluorescent microplate reader. The Oris Cell Migration Assay format allows researchers to capture more data per well using a number of instrument platforms to monitor cell movement.
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