ASB Launches Mouse Promoter Array (M8K) for its ChIP-GLAS Assay

Aviva Systems Biology (ASB) has announced the immediate availability of mouse promoter array system, M8K ChIP- GLAS (Chomatin Immunoprecipitation-Guided Ligation and Selection).

M8K ChIP- GLAS products are designed to provide an unprecedented degree of assay sensitivity and specificity for customers mapping promoter/transcription factor interactions or determining DNA methylation status examining over 8000 mouse promoters simultaneously.

To validate the mouse promoter array, mouse DNA from tissues or cells that is bound to transcription factors or hypermethylated is captured, either by a TF protein-specific antibody or a DNA modification-specific antibody (such as ASB's methylation-specific anti-5-methylcytosine monoclonal antibody).

A feature of the ChIP-GLAS system is the GLAS (Guided Ligation and Selection) step. In this step the DNA of interest is selected by annealing to oligonucleotides corresponding to promoters on the microarray.

This step is designed to decrease the sample complexity, thereby increasing both the sensitivity and the specificity of ChIP-GLAS compared to similar techniques.

The annealing step is followed by PCR amplification, labeling, and hybridization to a promoter microarray.

"This new mouse promoter array highlights the solid R&D efforts by ASB," stated Mr. Julian Yuan, CEO of Aviva Systems Biology.

"Each mouse promoter region was carefully annotated by our bioinformatics team, and also experimentally validated for its accuracy. We are committed to offering the highest quality products to our customers."

"Together with the release of over 1000 new antibodies for mouse and human transcription factors in 2006 and continuous production of antibodies to all known and putative transcription factors, we are excited to have mouse product for both basic research and drug discovery applications."

ChIP-GLAS technology was invented in the laboratory of Dr. Xiangdong Fu, a Professor at UC San Diego, and licensed exclusively by Aviva Systems Biology.

This technology was also selected in the NIH ENCODE (ENCyclopedia Of DNA Elements) development project to identify functional DNA elements within the human genome.

Based on the ChIP-GLAS technology, the 20,000 the human promoter array (H20K) was developed and launched in 2005.