We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Dolomite Aids Synthetic Biology Research
Product News

Dolomite Aids Synthetic Biology Research

Dolomite Aids Synthetic Biology Research
Product News

Dolomite Aids Synthetic Biology Research


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "Dolomite Aids Synthetic Biology Research"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Dolomite microfluidic chips are helping researchers from the Biodesign Institute at Arizona State University (ASU) to develop novel enzymes capable of polymerising synthetic nucleotides. Using these chips, the team has created a droplet-based optical polymerase sorting (DrOPS) technique allowing rapid screening for novel polymerase activities in uniform water-in-oil microcompartments. The team’s leader, Professor John C. Chaput – formerly at ASU and currently at the University of California, Irvine – explained: “The creation of synthetic nucleic acids is of great interest to synthetic biologists but, because they are not found in nature, wild type polymerases struggle to process them. To overcome this issue, we are developing novel polymerases using directed evolution in water-in-oil microcompartments. The DrOPS methodology has significant advantages over traditional methods, which are both labour intensive and impractical to perform on a large scale due to the amount of precious artificial nucleotide reagents required for screening.”

The Biodesign Institute turned to microfluidics to allow rapid sorting and screening of novel polymerases, taking advantage of the technique’s single-cell encapsulation capabilities and picolitre reaction volumes. Dr Andrew Larsen commented: “We needed very reproducible microfluidics, and so using commercially available chips was preferable. We already had experience with Dolomite’s chips for a variety of applications within the institute, and they have always been very consistent, so the choice was obvious. These chips give us the ability to consistently generate uniform droplets – both single and double emulsion droplets – offering spatial separation between cells and allowing fluorescence-based sorting using conventional FACS technologies. Dolomite has also been very supportive of our efforts, helping to accelerate this area of research.”

Advertisement