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Dolomite’s Microfluidics Technology Ideal for B Cell Encapsulation


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Associate Professor Moonsoo Jin from the Department of Radiology explained: “Some of our immunology research is focused on cloning antibody genes. In this work, it is important to ensure that individual genes are isolated from each other, and that is why we have adopted the droplet technology. It enables us to encapsulate a single human or mouse B cell, guaranteeing that antibody genes from one cell are not mixed with those from another cell.”

 “Biology itself is difficult enough, and so when we needed a microfluidic system for a new project we looked for a droplet generation system that was straightforward to use and not too complex. We chose Dolomite, investing in Mitos P-Pumps and a quartz Droplet Junction Chip. We’ve had the system for about two years now, and our experience has been very positive. It is simple to use and reliable, and we've been able to generate stable, uniform-size droplets able to withstand subsequent molecular biology procedures in a very reproducible and robust manner. We plan to continue our work with B cells and, in the future, may also use the droplet generation system to look at T cells and T cell receptor diversity and specificity.”

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