New Lab901 Technical Note: The ScreenTape Degradation Value (SDV), a new standard for RNA QC
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Lab901 has announced the availability of a new technical note entitled ‘The ScreenTape® Degradation Value (SDV), a new standard for RNA QC.’ The technical note can be downloaded from http://www.lab901.net/images/pdfs/lmm-041-pdf.pdf. Lab901’s ScreenTape Degradation Value (SDV)1 is an RNA QC metric that is specifically optimised for, and fully integrated with, Lab901’s ScreenTape R6K platform.
In an independent benchmarking study, the SDV has been shown to be equivalent to the RIN number for total RNA quality assessment and to offer better discrimination for poorer quality samples. Lab901’s software calculates RNA degradation based on factors that avoid common difficulties associated with the interpretation of RNA electropherograms.
Generation of the SDV is independent of ribosomal peak volumes and therefore avoids the inherent uncertainty of relying on peak boundaries. The result is an RNA QC metric that can reproducibly help researchers decide which samples can be used for downstream microarray, next generation sequencing and RT-qPCR experiments.
In an independent benchmarking study, the SDV has been shown to be equivalent to the RIN number for total RNA quality assessment and to offer better discrimination for poorer quality samples. Lab901’s software calculates RNA degradation based on factors that avoid common difficulties associated with the interpretation of RNA electropherograms.
Generation of the SDV is independent of ribosomal peak volumes and therefore avoids the inherent uncertainty of relying on peak boundaries. The result is an RNA QC metric that can reproducibly help researchers decide which samples can be used for downstream microarray, next generation sequencing and RT-qPCR experiments.
