Next Generation of Microarray Substrates
Product News Jan 26, 2006
The Nexterion® HiSens, (High Sensitivity), slides are designed to increase the signal to noise ratios from microarray experiments by over eight times and were developed using the company’s optical coating technology.
br/>Most microarray users prefer to use fluorescent dyes as labels in their experiments, as their use enables detection of two or more different dyes simultaneously. Unfortunately, microarray scanners do not operate at their maximum efficiency as they only acquire a small proportion of the total fluorescence emitted from the spots on conventional slides.
br/>The Nexterion® HiSens reflective coating overcomes this limitation. According to SCHOTT, Nexterion® HiSens is the ultimate microarray slide for microarray applications where the target material is at a low concentration or cannot be reliably amplified, such as applications measuring mRNAs or low abundant proteins.
br/>The Nexterion® HiSens coating produces reliable and reproducible signals from low expressor genes. The HiSens slides are manufactured with either a high quality Epoxy or Aminosilane functional coating, under class 100 clean room conditions in a state of the art production facility.
br/>Nexterion® HiSens slides can are fully compatible with all microarray printing technologies and most common slide processing protocols. Nexterion® HiSens is based on an ultra-flat, high quality borosilicate glass slide coated with a reflective optical layer.
br/>The reflective layer on Nexterion® HiSens enhances sensitivity and signal response by a process of fluorescence enhancement and reflection of the emitted light. The characteristics of the reflective layers have been tuned to the fluorescent wavelengths most commonly used in microarray experiments, and will simultaneously enhance the signal in both channels.